Reporter

Part:BBa_K1114207:Design

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)


CyPet_CD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 218
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 218
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 648


Design Notes

This is the MoClo formatted part of Cypet with CD fusion sites. This is a Level 0 MoClo part with flanking sites C on the 5' side and site D on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See pSB1C3.

Source

evan appleton

References